yhdW [2019-08-27 10:18:39]

similar to glycerophosphodiester phosphodiesterase

locus: BSU_09620

pI: 9.54

mw: 27.33 kDa

protein length: 243 aa

gene length: 732 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.1|Utilization of lipids] → [category|SW 2.4.1.2|Utilization of phospholipids/ based on similarity]

Gene

Coordinates: 1,037,688 1,038,419

The protein

Catalyzed reaction/ biological activity

• sn-glycero-3-phosphoester + H₂O --> alcohol + H⁺ + sn-glycerol 3-phosphate (according to UniProt)

Protein family

• glycerophosphoryl diester phosphodiesterase family (with [protein|E9BEF368F8E55888AD4849E8906E517831A882DF|GlpQ], according to UniProt)

Paralogous protein(s)

• [protein|AF37A0E08AAAA9159DD1F0183A61713EF582E830|YqiK], [protein|E9BEF368F8E55888AD4849E8906E517831A882DF|GlpQ]

Structure

• [PDB|5T9B] ([protein|E9BEF368F8E55888AD4849E8906E517831A882DF|GlpQ], 38% identity) [pubmed|27780866]

Expression and Regulation

Operons

• genes: [gene|04CACAF4F786090AB67D7C606C944F07B7A7FE55|yhdW]-[gene|BD1C4D9E6E8960DF77EE1571A637929C2D8C4A28|yhdV]-[gene|2F86A2FF36ED00DB0A9A91B064EBD095F5E759ED|fluC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B487 (yhdW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1486 NBRP B. subtilis, Japan]
• BKE09620 ([gene|04CACAF4F786090AB67D7C606C944F07B7A7FE55|yhdW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCTAAGTCAGCTCCTCG, downstream forward: _UP4_GATTTCATCATAAAGGATGG
• BKK09620 ([gene|04CACAF4F786090AB67D7C606C944F07B7A7FE55|yhdW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCTAAGTCAGCTCCTCG, downstream forward: _UP4_GATTTCATCATAAAGGATGG

References

Research papers

• 27780866